Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 55, Issue 16, Pages 6700-6704Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf070805r
Keywords
flavor authenticity; alpha-ionone; beta-ionone; alpha-ionol; IRMS; HRGC-C/P-IRMS; C-13/C-12 ratio; H-2/H-1 ratio; raspberry (Rubus idaeus)
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In addition to the already available information on the authenticity of alpha- (1) and beta-ionone (2) from plant tissues, there is an interest in the stable isotope data of 1 and 2 available by synthesis from citral and acetone, as European Union regulations, in contrast to the United States and other countries, do not allow a product to be declared as 'natural' that has been chemically synthesized (e.g., by using a natural catalyst) from natural educts. Analyses performed by on-line capillary gas chromatography-isotope ratio mass spectrometry in the combustion and pyrolysis modes (HRGC-C/P-IRMS) as well as by elemental analyzers (EA-C/P-IRMS) measuring delta C-13(V-PDB) and delta H-2(V-SMOW) values provide for the first time isotope data of such 'natural' 1 and 2 as well as of synthetic and 'ex plant' alpha-ionol (3). The isotope data recorded for synthesized 1 and 2 reflected the influence of the origin of the used citral, whereas that of acetone was less remarkable. For instance, 'natural' 1 ex citral from lemongrass showed, as expected for a C4 plant, an enriched delta C-13(V-PDB) value of -18.5%. In addition, the use of synthetic citral resulted in an enriched delta H-2(V-SMOW) value of -43%, whereas with citral ex Litsea cubeba and ex lemongrass values of -242 and -232%, respectively, were recorded. IRMS analyses of 'natural' 2 revealed delta C-13(V-PDB) and delta H-2(V-SMOW) values that were nearly identical to that recorded for 'natural' 1. As to both 1 and 2, variations of synthesis conditions led to distinct changes in the delta C-13(V-PDB) but not the delta H-2(V-SMOW) values. Synthetic 3 showed delta C-13(V-PDB) and delta H-2(V-SMOW) values of -24.5 and -184%, respectively. These data differed from those found in raspberry fruit under study (n = 8), that is, ranging from -33.6 to -36.6% for delta C-13(V-PDB) and from -200 to -225% for delta H-2(V-SMOW). The values determined additionally for 1 and 2 in raspberry fruit samples ranged from -30.3 to -35.1% and from -176 to -221% for delta C-13(V-PDB) and delta H-2(V-SMOW), respectively, and thus corresponded to the already known literature information.
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