Journal
LIFE SCIENCES
Volume 81, Issue 9, Pages 695-701Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2007.05.028
Keywords
prostate cancer; IFN gamma; gene therapy; adenoviral vector
Ask authors/readers for more resources
Interferon gamma (IFN-gamma) is regarded as a potent antitumor agent, but therapy with IFN-gamma is hampered by its short half-life and significant side effects. We developed a replication defective adenovirus carrying the human IFN-gamma gene and evaluated the effects of adenovirus-mediated IFN-gamma (Ad-IFN-gamma) gene transfer on human prostate cancer cell lines in vitro and on xenografts in vivo. Our results showed infection of prostate cancer cells with Ad-IFN-gamma led to production of an active cytokine and resulted in an antiproliferative effect on the prostate cancer cells. Intraturnoral injection of Ad-IFN-gamma significantly inhibited the growth of DU-145 cell xenografts in vivo, while no significant toxicity effect was observed. RT-PCR analysis indicated transgene expression mainly enriched in tumors in vivo, and slightly distributed in livers. These findings suggest adenovirus-mediated IFN-gamma gene transfer is a promising approach in the treatment of advanced prostate cancer. (c) 2007 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available