Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1160, Issue 1-2, Pages 270-275Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.05.047
Keywords
flow field-flow fractionation; FFF; gamma radiation; sodium hyaluronate; molecular weight distribution; conformation of sodium hyaluronate; field programming
Funding
- National Research Foundation of Korea [R11-2003-019-01001-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A combined flow field-flow fractionation (FIFFF)/multiangle light scattering (MALS)/differential refractive index (DRI) detection method has been utilized for the size fractionation and characterization of ultrahigh molecular weight (MW) sodium hyaluronate (NaHA) samples. Separation of broad MW NaHA polymers was carried out by a frit inlet asymmetrical FIFFF channel employed with a linear field programming method followed by the on-line monitoring of light scattering at multiple angles for the calculation of MW and for the study of the conformation of NaHA samples. NaHA samples examined were: (1) two different viscosity fractions of NaHA obtained by a refinement process and (2) NaHA products of gamma radiation degradation. While the NaHA samples of two different viscosity fractions exhibited clearly different MW distributions and similar conformation, the radiation degraded NaHA samples showed a clear difference in both MW distribution and polymer structure. (c) 2007 Elsevier B.V. All rights reserved.
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