Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1160, Issue 1-2, Pages 114-119Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.04.015
Keywords
polymer monolith microextraction; in situ derivatization; hexanal; heptanal; 2,4-dinitrophenylhydrazine (DNPH); HPLC
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A simple, rapid and sensitive method for the determination of hexanal and heptanal in plasma by high-performance liquid chromatography (HPLC) has been developed, which is based on polymer monolith microextraction (PMME) with in situ derivatization. 2,4-dinitrophenylhydrazine (DNPH) as a derivatizing reagent was first adsorbed on a poly (methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith, and then microextraction was performed simultaneously with derivatization on the monolith. The several parameters affecting the in situ derivatization simultaneously with PMME were investigated, including the flow rate, pH, buffer concentration, and temperature. The whole pretreatment process can be accomplished within 8 min. The limits of detection for hexanal and heptanal were found to be 2.4 and 3.6 nmol/L, respectively. The recoveries in plasma sample were in the range of 83-87% with the inter- and intra-day precisions less than 6.8%. This method was successfully applied to the analysis of hexanal and heptanal in plasma samples from different cancer patients. (c) 2007 Elsevier B.V. All rights reserved.
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