Focused-ion-beam-fabricated Au nanorods coupled with Ag nanoparticles used as surface-enhanced Raman scattering-active substrate for analyzing trace melamine constituents in solution

A well-ordered Au-nanorod array with a controlled tip ring diameter (Au_NRs(d)) was fabricated using the focused ion beam method. Au_NRs(d) was then coupled with Ag nanoparticles (Ag NPs) to bridge the gaps among Au nanorods. The effect of surface-enhanced Raman scattering (SERS) on Au_NRs(d) and Ag NPs/Au_NRs(d) was particularly verified using crystal violet (CV) as the molecular probe. Raman intensity obtained from a characteristic peak of CV on Au_NRsd was estimated by an enhancement factor of approximate to 10(7) in magnitude, which increased approximate to 10(12) in magnitude for that on Ag NPs/Au_NRs(d). A highly SERS-active Ag NPs/Au_NRs(d) was furthermore applied for the detection of melamine (MEL) at very low concentrations. Raman-active peaks of MEL (10 (3) to 10 (12) M) in water or milk solution upon Au_NRs(d) or Ag NPs/Au_NRs(d) were well distinguished. The peaks at 680 and 702 cm(-1) for MEL molecules were found suitable to be used as the index for sensing low-concentration MEL in a varied solution, while that at 1051 cm(-1) was practical to interpret MEL molecules in water or milk solution bonded with Au (i.e., Au_NRs(d)) or Ag (i.e., Ag NPs/Au_NRs(d)) surface. At the interface of Ag NPs/Au_NRs(d) and MEL molecules in milk solution, a laser-induced electromagnetic field or hotspot effect was produced and competent to sense low-concentration MEL molecules interacting with Ag and Au surfaces. Accordingly, Ag NPs/Au_NRs(d) is very promising to be used as a fast and sensitive tool for screening MEL in complex matrices such as adulteration in e. g., food and pharmaceutical products. (C) 2013 Elsevier B. V. All rights reserved.