Journal
CARBOHYDRATE RESEARCH
Volume 342, Issue 11, Pages 1442-1449Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2007.03.031
Keywords
uronic acid; carbazole; methanolysis; 4-O-methylglucuronic acid; galacturonic acid; glucuronic acid; hexenuronic acid; xylan
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Uronic acids can be quantified either by a colorimetric determination after treatment with concentrated sulfuric acid and carbazole or by gas chromatography after methanolysis and subsequent acetylation. Both methods suffer from incomplete hydrolysis, an unavoidable degradation of the products to be analysed, and an inability to separate and quantify different types of uronic acids. In the present work, the fundamental chemistry involved in the two methods has been evaluated, and some modifications to increase their accuracy are suggested. By combining the two methods, a complete quantification of all individual types of urome acids present in a sample can be achieved. (c) 2007 Elsevier Ltd. All rights reserved.
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