Journal
VACCINE
Volume 25, Issue 33, Pages 6224-6231Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2007.05.065
Keywords
rotavirus; VP6; immunity
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Intranasal or oral delivery of the chimeric rotavirus VP6 protein MBP::VP6 to mice elicited > 90% reductions in fecal rotavirus shedding after murine rotavirus challenge. Protection depended on co-admirristration of adjuvants, the most effective being bacterial toxins. Because of safety and efficacy concerns following intranasal or oral toxin delivery, protective efficacy of MBP::VP6 after intrarectal delivery with toxin adjuvants was determined and compared to that induced after intranasal and oral immunization. Adult BALB/c mice were orally challenged with the murine rotavirus strain EDIM 4 weeks after their second immunization with MBP::VP6 and either LT(R192G), an attenuated Escherichia coli heat-labile toxin, or CTA1-DD, a cholera toxin derivative. Reductions in fecal rotavirus shedding were then determined relative to mockimmunized mice. Immunization with MBP::VP6 and either adjuvant by any route (except oral immunization with CTA1-DD) significantly (P < 0.0001) reduced rotavirus shedding. As was previously found after oral and intranasal immunization, intrarectal immunization with MBP::VP6 and adjuvant was associated with T cell responses (IFN gamma and IL-17) but not B cell (antibody) responses. (c) 2007 Elsevier Ltd. All rights reserved.
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