Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 33, Pages 13396-13401Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0701965104
Keywords
meiosis; mouse strain; recombination; single-nucleotide polymorphism; allele-specified PCR
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Funding
- NICHD NIH HHS [R01 HD040916, HD 40916] Funding Source: Medline
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Fertility requires successful chromosome segregation in meiosis, which in most sexual organisms depends on the formation of appropriately placed crossovers. The nonrandom genome-wide distributions of meiotic recombination events have been examined at the molecular level experimentally in yeast and by inference from linkage disequilibrium patterns in humans. Thus far, no method has existed for pinpointing sites of crossing-over on a genome-wide scale in an experimentally tractable animal whose genome size and complexity models that of humans. Here, we present a genomic approach to identify mouse crossover hotspots, based on targeting haplotype block boundaries. This represents a previously undescribed method potentially applicable to largescale mouse hotspot identification. Using this method, we have successfully predicted the location of two previously uncharacterized crossover hotspots in male mice. As increasing amounts of sing le-nucleotide polymorphism data emerge, this approach will be useful for investigating the recombination landscape of the mouse genome.
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