Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 33, Pages 13349-13354Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0705889104
Keywords
chemoattractant; gradient; neutrophils
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Funding
- NIGMS NIH HHS [R01 GM027800, R37 GM027800, GM 27800] Funding Source: Medline
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We have analyzed chemotaxis of neutrophil-differentiated HL60 cells in microfluidic devices that create exponential gradients of the chemoattractant, f-Met-Leu-Phe (fMLP). Such gradients expose each cell to a difference in fMLP concentration (Delta C) across its diameter that is directly proportional to the ambient concentration (C) at that cell's position in the gradient, so the ratio Delta C/C is constant everywhere. Cells exposed to ambient fMLP concentrations near the constant of dissociation (K-d) for fMLP binding to its receptor (approximate to 10 nM) crawl much less frequently when Delta C/C is 0.05 than when it is 0.09 or 0.13. Hence, cells can detect the gradient across their diameter without moving and, thus, without experiencing temporal changes in attractant concentration. At all Delta C/C ratios tested, the average chemotactic prowess of individual cells (indicated by the distance a cell traveled in the correct direction divided by the length of its migration path) is maximal for cells that start migrating at concentrations near the Kd and progressively decreases at higher or lower starting concentrations.
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