Journal
NEUROIMAGE
Volume 37, Issue 2, Pages 530-538Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.neuroimage.2007.04.067
Keywords
neural tracer; DTI tractography; histology; 3D reconstruction
Funding
- NCRR NIH HHS [P41 RR013218, R01 RR021885, U41 RR019703] Funding Source: Medline
- NEI NIH HHS [P30-EY12196, R01 EY011379] Funding Source: Medline
- NHLBI NIH HHS [R01 HL074942] Funding Source: Medline
- NIBIB NIH HHS [U54 EB005149] Funding Source: Medline
- NICHD NIH HHS [P01 HD18655] Funding Source: Medline
- NIMH NIH HHS [R01-MH50747, R21 MH067054] Funding Source: Medline
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Since the introduction of diffusion weighted imaging (DWI) as a method for examining neural connectivity, its accuracy has not been formally evaluated. In this study, we directly compared connections that were visualized using injected neural tract tracers (WGA-HRP) with those obtained using in-vivo diffusion tensor imaging (DTI) tractography. First, we injected the tracer at multiple sites in the brain of a macaque monkey; second, we reconstructed the histological sections of the labeled fiber tracts in 31); third, we segmented and registered the fibers (somatosensory and motor tracts) with the anatomical in-vivo MRI from the same animal; and last, we conducted fiber tracing along the same pathways on the DTI data using a classical diffusion tracing technique with the injection sites as seeds. To evaluate the performance of DTI fiber tracing, we compared the fibers derived from the DTI tractography with those segmented from the histology. We also studied the influence of the parameters controlling the tractography by comparing Dice superimposition coefficients between histology and DTI segmentations. While there was generally good visual agreement between the two methods, our quantitative comparisons reveal certain limitations of DTI tractography, particularly for regions at remote locations from seeds. We have thus demonstrated the importance of appropriate settings for realistic tractography results. (c) 2007 Elsevier Inc. All rights reserved.
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