Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 16, Pages 6410-6418Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac070585g
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Funding
- NCRR NIH HHS [P41 RR011823, P41 RR11823-10, P41 RR011823-10] Funding Source: Medline
- NIMH NIH HHS [R01 MH067880, R01 MH067880-06] Funding Source: Medline
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Multidimensional liquid chromatography in combination with tandem mass spectrometry has been used to analyze a variety of biological structures including protein complexes. Incorporating this approach with autosampling devices presents a number of problems including decreased sensitivity due to exposure to extra surfaces, carryover from run to run, and increased dead volume. We developed a device, termed Radial Column Array for Distribution and Automation (RCADiA), to automate multiple MuDPIT experiments while eliminating many of these problems and maintaining a high resolution and sensitive analysis. The design, which places each sample downstream of any common fluid path, presents a low risk of carryover between successive analyses. Beyond the convenience of automation, the RCADiA platform also produces data of similar quality to the standard method of performing individual MuDPIT experiments. We demonstrate this device by performing a comparative analysis of mitochondria enriched from rat liver and spinal cord.
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