Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 583, Issue 1, Pages 337-350Publisher
BLACKWELL PUBLISHING
DOI: 10.1113/jphysiol.2007.135426
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Funding
- NHLBI NIH HHS [HL65497, HL61683, R01 HL065497, R01 HL061683, 1K99HL087462-01, K99 HL087462] Funding Source: Medline
- NIBIB NIH HHS [T32 EB001650] Funding Source: Medline
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We studied how enhanced skeletal troponin C (sTnC) Ca2+-binding affinity affects cooperative thin filament activation and contraction in single demembranated rabbit psoas fibres. Three sTnC mutants were created and incorporated into skeletal troponin (sTn) for measurement of Ca2+ dissociation, resulting in the following order of rates: wild-type (WT) sTnC-sTn > sTnC(F27w)-sTn > M80Q sTnC-sTn > M80Q sTnC(F27w)-sTn. Reconstitution of sTnC-extracted fibres increased Ca2+ sensitivity of steady-state force (pCa(50)) by 0.08 for M80Q sTnC, 0.15 for sTnC(F27w) and 0.32 for M80Q sTnC(F27W) with minimal loss of slope (n(H), degree of cooperativity). Near-neighbour thin filament regulatory unit (RU) interactions were reduced in fibres by incorporating mixtures of WT or mutant sTnC and D28A, D64A sTnC (xxsTnC) that does not bind Ca2+ at N-terminal sites. Reconstitution with sTnC: xxsTnC mixtures to 20% of pre-exchanged maximal force reduced pCa(50) by 0.35 for sTnC: xxsTnC, 0.25 for M80Q sTnC: xxsTnC, and 0.10 for M80Q sTnC(F27W): xxsTnC. It is interesting that pCa(50) increased by similar to 0.1 for M80Q sTnC and similar to 0.3 for M80Q sTnC(F27W) when near-neighbour RU interactions were reduced; these values are similar in magnitude to those for fibres reconstituted with 100% mutant sTnC. After reconstitution with sTnC: xxsTnC mixtures, nH decreased to a similar value for all mutant sTnCs. Altered sTnC Ca2+-binding properties (M80Q sTnC(F27W)) did not affect strong crossbridge inhibition by 2,3-butanedione monoxime when near-neighbour thin filament RU interactions were reduced. Together these results suggest increased sTnC Ca2+ affinity strongly influences Ca2+ sensitivity of steady-state force without affecting near-neighbour thin filament RU cooperative activation or the relative contribution of crossbridges versus Ca2+ to thin filament activation.
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