Journal
ANALYTICA CHIMICA ACTA
Volume 767, Issue -, Pages 148-154Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.01.001
Keywords
Ionic liquids; Mobile phase additives; Stephania tetrandra S. Moore; Reversed phase high performance liquid chromatography
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Funding
- Natural Science Foundation of China [31170110]
- Natural Science Foundation of Shandong Province [2009ZRB01753]
- Key National Science & Technology Specific Projects of China [2010ZX09401-302-5-12]
- Key Technologies R&D Program of Shandong Province [2010GWZ20243]
- Project of Taishan Scholarship of Shandong Province
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A reversed phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of fangchinoline (FAN) and tetrandrine (TET) in Stephania tetrandra S. Moore was established by using 1-hexyl-3-methylimidazolium tetrafluoroborate as the mobile phase additives in this paper. Four types of 1-alkyl-3-methylimidazolium-based ionic liquids (ILs) were used as additives of the mobile phase to separate FAN and TET by RP-HPLC. The effects of the length of the alkyl group on the imidazolium ring and its counterion, the concentrations of IL and the pH of the mobile phase, which influenced the chromatographic behaviors of FAN and TET, were investigated in detail. The linearity, sensitivity, accuracy and repeatability of the proposed method were also investigated. The probable mechanism of the separation with ILs as the mobile phase additives was explored and discussed. (C) 2013 Elsevier B.V. All rights reserved.
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