Journal
ANALYTICA CHIMICA ACTA
Volume 771, Issue -, Pages 37-41Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2013.02.009
Keywords
Dye-doped core-shell silica nanoparticles; Magnetic nanoparticles; Laser-induced fluorescence; Determination of enrofloxacin; Antibiotics; Sample treatment using nanoparticles
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Funding
- Korea Food and Drug Administration [10162KFDA995]
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A unique analytical method using nanoparticles and laser-induced fluorescence microscopy (LIFM) was developed to determine enrofloxacin in this work. For sample pretreatment, two different kinds of particles, i.e., synthesized dye-doped core-shell silica nanoparticles and magnetic micro-particles (MPs), were used for fluorescent tagging and concentrating the enrofloxacin, respectively. The antibody of enrofloxacin was immobilized on the synthesized FITC-doped core-shell nanoparticles, and the enrofloxacin target was extracted by the MPs. At this moment, the average number of antibodies on each core-shell silica nanoparticle was similar to 0.9, which was determined by the fluorescence ratiometric method. The described method was demonstrated for a meat sample to determine enrofloxacin using LIFM, and the result was compared with enzyme-linked immunosorbent assay (ELISA). The developed technique allowed the simplified analytical procedure, improved the detection limit about 54-fold compared to ELISA. (C) 2013 Elsevier B.V. All rights reserved.
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