Journal
ANALYTICA CHIMICA ACTA
Volume 761, Issue -, Pages 194-200Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.11.046
Keywords
Mesoporous silica nanoparticle; Ultrabright fluorescence; Fluorescent label; Chemoluminescent label; Fluorescence intensity; Photostablity
Categories
Funding
- Fundamental Research Funds for the Central Universities [GK20091004]
- National High-tech R&D Program (863 Program) of China [2006AA02A237]
- National Natural Science Foundation of China [30872371, 30901358]
Ask authors/readers for more resources
A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90 nm silica particles was about 61%. Compared with the fluorescent core-shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO4 oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available