4.8 Article

A thiol-reactive fluorescence probe based on donor-excited photoinduced electron transfer: Key role of ortho substitution

Journal

ORGANIC LETTERS
Volume 9, Issue 17, Pages 3375-3377

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ol071352e

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We designed and synthesized a novel thiol-reactive fluorescence probe based on the BODIPY fluorophore. The fluorescence of this probe is strongly quenched by donor-excited photoinduced electron transfer (d-PeT) from BODIPY to maleimide, but after reaction with thiol, the fluorescence of BODIPY is restored, affording a 350-fold intensity increase.

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