Journal
ANALYTICA CHIMICA ACTA
Volume 753, Issue -, Pages 57-63Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.09.028
Keywords
Hydroxylated polycyclic aromatic hydrocarbons; Accelerated solvent extraction; Subcritical water extraction; Dispersive liquid-liquid microextraction; Sediment
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Funding
- National Natural Science Foundation of China (NSFC) [20977116, 21077139, 21277177]
- Fundamental Research Funds for the Central Universities
- Specialized Research Fund for the Doctoral Program of Higher Education [0090171110041]
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A sample preparation method for the determination of hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in sediment samples was developed using gas chromatography-mass spectrometry (GC-MS). Dispersive liquid-liquid microextraction (DLLME) with derivatization was performed following the sub-critical water extraction (SWE) that provided which was provided by accelerated solvent extraction (ASE). Several important parameters that affected both SWE extraction and DLLME, such as the selection of organic modifier, its volume, extraction temperature, extraction pressure and extraction time were also investigated. High sensitivity of the hydroxylatecl PAHs derivatives by N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) could be achieved with the limits of detection (LODs) ranging from 0.0139 (2-OH-nap) to 0.2334 mu g kg(-1) (3-OH-fluo) and the relative standard deviations (RSDs) between 2.81% (2-OH-phe) and 11.07% (1-OH-pyr). Moreover, the proposed method was compared with SWE coupled with solid phase extraction (SPE). and the results showed that ASE-DLLME was more promising with recoveries ranging from 57.63% to 91.07%. The proposed method was then applied to determine the hydroxylated metabolites of phenanthrene in contaminated sediments produced during the degradation by two PAH-degraders isolated from mangrove sediments. (C) 2012 Elsevier B.V. All rights reserved.
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