Journal
ANALYTICA CHIMICA ACTA
Volume 747, Issue -, Pages 76-83Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.08.038
Keywords
Proton nuclear magnetic resonance; Rhizoma coptidis; Protoberberine alkaloids; Quantitative analysis; Species differentiation
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Funding
- Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-Year Plan Period of China [2007BAI40B05, 2007BAI40B03]
- Science Development Fund of Chengdu University of Traditional Chinese Medicine [ZRZD201103]
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Rhizoma coptidis, a broadly used traditional Chinese medicine, derives from the dried rhizomes of Coatis chinensis Franch, Coptis deltoidea C.V. Cheng et Hsiao and Coptis teeta Wall. Quantitative determination of protoberberine alkaloids in R. coptidis is critical for controlling its quality. In this study, a rapid, simple and accurate quantitative H-1 NMR (qNMR) method was developed for simultaneous determination of berberine, jatrorrhizine, epiberberine, coptisine, palmatine and columbamine in R. coptidis from the three species. Method validation was performed in terms of selectivity, precision, repeatability, stability, accuracy, robustness and linearity. The average recoveries obtained were in the range of 96.9-102.4% for all the six alkaloids. In addition, the qNMR data were analyzed with analysis of variance (ANOVA), hierarchical clustering analysis (HCA) and principal component analysis (PCA), and the results showed that the contents of the active alkaloids have significant difference among the three species. Compared with the conventional HPLC approach, the proposed qNMR method was demonstrated to be a powerful tool for quantifying the six alkaloids due to its unique advantages of high robustness, rapid analysis time and no need of standard compounds for calibration curves preparation. These findings indicate that this method has potential as a reliable method for quality evaluation of herb medicines, especially for protoberberine alkaloid-containing ones. (C) 2012 Elsevier B.V. All rights reserved.
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