Journal
ANALYTICA CHIMICA ACTA
Volume 709, Issue -, Pages 41-46Publisher
ELSEVIER
DOI: 10.1016/j.aca.2011.10.008
Keywords
Enzyme; Microreactor; Electrochemical detection; Magnetic nanoparticles; Glucose
Categories
Funding
- National Science Fund for Creative Research Groups [20821063]
- National Natural Science Foundation of China [20875044, 21075055, 21005070]
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A microfluidic device was designed for amperometric determination of glucose by packing enzyme modified magnetic nanoparticles (MNPs) in its microchannel as an enzyme microreactor. Glucose oxidase was covalently attached to the surface of MNPs and localized in the microchannel by the help of an external magnetic field, leading to a tunable packing length. By changing the length of microreactor from 3 to 10mm, the performance for glucose detection was optimized. The optimal linear range to glucose was from 25 mu M to 15 mM with a detection limit of 11 mu M at a length of 6 mm. The inter- and intra-day precisions for determination of 1.0 mM glucose were 0.8% and 1.7%, respectively, and the device-to-device reproducibility was 95.6%. The enzyme reactor remained its 81% activity after three-week storage. Due to the advantages of the device and fracture sampling technique, serum samples could be directly sampled through the fracture to achieve baseline separation from ascorbic acid, and proteins in the samples did not interfere with the detection. This work provided a promising way for pretreatment-free determination of glucose with low cost and excellent performance. (C) 2011 Elsevier B.V. All rights reserved.
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