Journal
ANALYTICA CHIMICA ACTA
Volume 749, Issue -, Pages 51-55Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2012.08.039
Keywords
Gonads; Prostaglandins; Prostaglandin E-2; Prostaglandin F-2a; Liquid chromatography-tandem mass; spectrometry; Atlantic cod
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Funding
- European Commission
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A simple extraction method for the analysis of PGE(2) and PGF(2a) in gonad samples from Atlantic cod and further quantification by using liquid chromatography-tandem mass spectrometry is proposed. The evaluation of the best solvent extraction conditions and the analytical performance parameters are reported. The method was highly selective for both prostaglandins and the calibration curves, based on the internal standard method, were linear between 5 and 1000 ng mL(-1) for PGE(2) and PGF(2a) with limits of detection of 1 ng mL(-1) and 1.5 ng mL(-1) and recovery values of 99.999 +/- 0.002 and 99.967 +/- 0.023 respectively. The homogenization of samples using liquid nitrogen combined with the developed extraction protocol can be implemented in different types of biological tissues. (C) 2012 Elsevier B.V. All rights reserved.
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