Journal
ANALYTICA CHIMICA ACTA
Volume 715, Issue -, Pages 105-112Publisher
ELSEVIER
DOI: 10.1016/j.aca.2011.12.014
Keywords
Competitive ELISA; Site heterology; Landsteiner's principle; Fungicide; Strobilurins; Food safety
Categories
Funding
- Ministerio de Educacion y Ciencia [AGL2006-12750-C02-01/02/ALI]
- FEDER
- Ramon y Cajal
- Spanish Ministerio de Ciencia e Innovacion
- European Social Fund
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Azoxystrobin is a modern strobilurin fungicide used around the world to combat prime diseases affecting highly valuable crops. Accordingly, residues of this chemical are frequently found in food, even though mostly under maximum tolerated levels. We herein describe the development of an indirect competitive immunoassay for the determination of azoxystrobin residues.,A panel of monoclonal antibodies displaying subnanomolar affinity to azoxystrobin was generated using, as immunizing haptens in mice, four functionalized derivatives carrying the same spacer arm located at different rationally chosen positions. This collection of antibodies was thoroughly characterized with homologous and heterologous antigens, and the immunoassay consisting of monoclonal antibody AZo6#49 and the coating conjugate OVA-AZb6, which displayed an IC50 value of 0.102 mu g L-1 and a LOD of 0.017 mu g L-1, was eventually optimized. The response to different pH and ionic strength conditions of the specific assay was studied using a biparametric approach. In addition, the influence of Tween 20 and organic solvents over the assay parameters was also evaluated. After optimization, the developed immunochemical assay was applied to the analysis of azoxystrobin in spiked juices of relevant fruits and vegetables, showing excellent recoveries between 2 and 500 mu g L-1. (C) 2011 Elsevier B.V. All rights reserved.
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