Journal
ANALYTICA CHIMICA ACTA
Volume 687, Issue 1, Pages 56-60Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.12.007
Keywords
Estrogens; Micro-solid-phase-extraction; Cancer risk; Cyst fluids; Ovarian cancer
Categories
Funding
- National University of Singapore (NUS)
Ask authors/readers for more resources
A cost effective and environmentally friendly extraction technique using porous membrane protected micro-solid phase extraction (mu-SPE) is described for the extraction of estrogens in cyst fluid samples obtained from cancer patients. A sorbent (ethylsilane (C2) modified silica) (20 mg) was packed in a porous polypropylene envelope (2 cm x 1.5 cm) whose edges were heat sealed to secure the contents. The mu-SPE device was conditioned with acetone and placed in a stirred (1:5) diluted cyst fluid sample solution (10 mL) to extract estrogens for 60 min. After extraction, the analytes were desorbed and simultaneously derivatized with a 5:1 mixture of acetone and N,O-bis(trimethylsilyl)-trifluoroacetamide. The extract (2 mu L) was analyzed by gas chromatography-mass spectrometry. Various extraction, desorption and derivatization conditions were optimized for mu-SPE. With this simple technique, low limits of detection of between 9 and 22 ng L-1 and linear range from the detection limits up to 50 mu g L-1 were achieved. The optimized method was used to extract estrogens from cyst fluid samples obtained from patients with malignant and benign ovarian tumors. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available