Journal
ANALYTICA CHIMICA ACTA
Volume 694, Issue 1-2, Pages 115-119Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2011.02.045
Keywords
Colorimetric detection; Mercury; DNA; Nanoparticles; Sensors
Categories
Funding
- National 264 High Technology Research and Development Program of China 265 (863 Program) [2007AA06A406]
- National Science 266 Foundation of China [20675031]
- Shanghai Science and Technology Committee [06PJ14032]
- Natural Science Foundation of Shanghai, China [11ZR1415400]
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A colorimetric nanoprobe-mercury-specific DNA-functionalized gold nanoparticles (Au-MSD) was developed for sensing Hg(2+). The new mercury-sensing concept relies on measuring changes in the inhibition of non-crosslinking aggregation of Au-MSD-induced by the folding of mercury-specific DNA strand through the thymine-Hg(2+)-thymine (T-Hg(2+)-T) coordination. In the absence of Hg(2+), a high concentration of MgCl(2) (50 mM) results in a rapid aggregation of Au-MSD because of the removal of charge repulsion. When Hg(2+) is present, the particles remain stable due to the folding of MSD functionalized on the particle surface. The assay enables the colorimetric detection of Hg(2+) in the concentration range of 0.1-10 mu M Hg(2+) ions with a detection limit of 60 nM, and allows for the selective discrimination of Hg(2+) ions from the other competitive metal ions. Toward the goal for practical applications, the sensor was further evaluated by monitoring Hg(2+) in fish tissue samples. (C) 2011 Elsevier B.V. All rights reserved.
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