Journal
ANALYTICA CHIMICA ACTA
Volume 685, Issue 1, Pages 84-90Publisher
ELSEVIER
DOI: 10.1016/j.aca.2010.11.007
Keywords
Capillary electrophoresis; Contactless conductivity detection; Photometric detection; Organic acids; Inborn errors of metabolism; Urine
Categories
Funding
- Grant Agency of the Czech Republic [P206/10/1231]
- Ministry of Education, Youth and Sports, Czech Republic [MSM0021620814]
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A mixture of 29 organic acids (OAs) occurring in urine was analyzed by capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection ((CD)-D-4) and UV photometric detection. The optimized analytical system involved a 100 cm long polyacrylamide-coated capillary (50 mu m i.d.) and the background electrolyte of 20 mM 2-morpholinoethanesulfonic acid (MES)/NaOH + 10% (v/v) methanol, pH 6.0 (pH is related to the 20 mM MES/NaOH buffer in water). The LOD values obtained by (CD)-D-4 for the OAs which do not absorb UV radiation range from 0.6 mu M (oxalic acid) to 6.8 mu M (pyruvic acid): those obtained by UV photometry for the remaining OAs range from 2.9 mu M (5-hydroxy-3-indoleacetic acid) to 10.2 mu M (uric acid). The repeatability of the procedure developed is characterized by the coefficients of variation, which vary between 0.3% (tartaric acid) and 0.6% (5-hydroxy-3-indoleacetic acid) for the migration time and between 1.3% (tartaric acid) and 3.5% (lactic acid) for the peak area. The procedure permitted quantitation of 20 OAs in a real urine sample and was applied to monitoring of the occurrence of the inborn metabolic fault of methylmalonic aciduria. (C) 2010 Elsevier B.V. All rights reserved.
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