Journal
ANALYTICA CHIMICA ACTA
Volume 701, Issue 2, Pages 218-223Publisher
ELSEVIER
DOI: 10.1016/j.aca.2011.06.018
Keywords
Phytohormone; Electrochemistry; Oxidase; Dehydrogenase; Quantitation; Electrode
Categories
Funding
- Ministry of Education Youth and Sports [MSM6198959216]
- European Regional Development Fund [CZ.1.05./2.1.00/01.0007]
- BBSRC [BB/F014651/1]
- BBSRC [BB/I023933/1, BB/F014651/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I023933/1, BB/F014651/1] Funding Source: researchfish
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An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N-6-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface. (C) 2011 Elsevier B.V. All rights reserved.
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