Journal
ANALYTICA CHIMICA ACTA
Volume 702, Issue 2, Pages 280-287Publisher
ELSEVIER
DOI: 10.1016/j.aca.2011.06.032
Keywords
Buprenorphine; Norbuprenorphine; Methadone; Naloxone; Liquid chromatography-coulometric detection; Microextraction by packed sorbent
Categories
Funding
- Dipartimento per le Politiche Antidroga (Italy), within the Farmagen
- VulCan
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The sublingual combination of buprenorphine and naloxone (Suboxone (R)) and Methadone Maintenance Therapy have been found effective in treating heroin addiction. A new analytical method suitable for the simultaneous determination of buprenorphine, norbuprenorphine, methadone and naloxone in human plasma by means of liquid chromatography with coulometric detection has been developed. The chromatographic separation was achieved with a phosphate buffer-acetonitrile mixture as the mobile phase on a cyano column. The monitoring cell of the coulometric detector was set at an oxidation potential of +0.600 V. A rapid clean-up procedure of the biological samples using a microextraction by packed sorbent technique has been implemented, employing a C8 sorbent inserted into a syringe needle. The extraction yield values were satisfactory for all analytes (>85%). The calibration curves were linear over a range of 0.25-20.0 ng mL(-1) for buprenorphine and norbuprenorphine, 3.0-1000.0 ng mL(-1) for methadone and 0.13-10.0 ng mL(-1) for naloxone. The sensitivity was also high with limits of detection of 0.08 ng mL(-1) for both buprenorphine and norbuprenorphine, 0.9 ng mL(-1) for methadone and 0.04 ng mL(-1) for naloxone. The intraday and interday precision data were always satisfactory. The method was successfully applied to plasma samples obtained from former heroin addicts treated with opioid replacement therapy. (C) 2011 Elsevier B.V. All rights reserved.
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