Journal
ANALYTICA CHIMICA ACTA
Volume 688, Issue 1, Pages 84-89Publisher
ELSEVIER
DOI: 10.1016/j.aca.2010.12.030
Keywords
Norfloxacin; Polyclonal antibody; Indirect competitive enzyme-linked immunosorbent assay; Liquid chromatography tandem mass spectrometry; Water samples
Categories
Funding
- National Water Project of China [2009ZX07527-005]
- NSFC [40972221]
- National Basic Research Program of China [2009CB421604]
Ask authors/readers for more resources
A specific polyclonal anti-norfloxacin antibody was obtained, and a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for determining trace amounts of norfloxacin in various waters. Good linearity was achieved in the range from 0.1 to 10 mu g L-1. The average IC50 value was determined to be 2.2 mu g L-1 and the limit of detection was 0.016 mu g L-1 at a signal-to-noise ratio of 3 in phosphate-buffered saline buffer. Recoveries of norfloxacin at various spiking levels ranged from 74 to 105% in groundwater, surface water, treated and untreated wastewater samples, with relative standard deviations of 3-5%. The assay was applied for determining norfloxacin in municipal wastewater, surface water, and groundwater collected in a metropolis of China. Raw wastewater samples were only submitted to filtration and pH adjustment while the other water samples were pre-concentrated by solid phase extraction prior to the icELISA assay. Good agreement of the results obtained by the icELISA and liquid chromatography tandem mass spectrometry further confirmed the reliability and accuracy of the icELISA for rapid detection of norfloxacin in waters. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available