Journal
ANALYTICA CHIMICA ACTA
Volume 675, Issue 2, Pages 148-155Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.07.024
Keywords
2-Acetyl-1-pyrroline; Convenient synthesis; SIDA; HS-SPME; GC-MS-MS; Rice
Categories
Funding
- Office National Interprofessionnel des Grandes Cultures (ONIGC)
- Languedoc Roussillon region
- Cirad
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A new and convenient synthesis of 2-acetyl-1-pyrroline (2AP), a potent flavor compound in rice, and its ring-deuterated analog, 2-acetyl-1-d(2)-pyrroline (2AP-d(2)), was reported. A stable isotope dilution assay (SIDA), involving headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-positive chemical ionization-ion trap-tandem mass spectrometry (GC-PCI-IT-MS-MS), was developed for 2AP quantification. A divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber was used for HS-SPME procedure and parameters affecting analytes recovery, such as extraction time and temperature, pH and salt, were studied. The repeatability of the method (n = 10) expressed as relative standard deviation (RSD) was 11.6%. A good linearity was observed from 5.9 to 779 ng of 2AP (r(2) = 0.9989). Limits of detection (LOD) and quantification (LOQ) for 2AP were 0.1 and 0.4 ngg(-1) of rice, respectively. The recovery of spiked 2AP from rice matrix was almost complete. The developed method was applied to the quantification of 2AP in aerial parts and grains of scented and non-scented rice cultivars. (C) 2010 Elsevier B.V. All rights reserved.
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