Journal
ANALYTICA CHIMICA ACTA
Volume 659, Issue 1-2, Pages 102-108Publisher
ELSEVIER
DOI: 10.1016/j.aca.2009.11.023
Keywords
Immunosensor; Au nanoparticles; Multi-walled carbon nanotubes; Chitosans; Carcinoembryonic antigen
Categories
Funding
- National Natural Science Foundation of China [20805040, 20875080]
- Natural Science Foundation of He'nan Province of China [082300420140]
- Foundation of He'nan Educational Committee [2008A150020]
- Program for Science&Technology Innovation Talents in Universities of Henan Province [2010HASTIT025]
Ask authors/readers for more resources
In this paper, a disposable electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) based on Au nanoparticles (AuNPs)/multi-walled carbon nanotubes (MWCNTs)-chitosans (Chits) composite film was developed. MWCNTs-Chits homogeneous composite was first dispersed in acetic acid solution and then the AuNPs was in situ synthesized at the composite. The mixture was dripped on the glassy carbon electrode (GCE) and then CEA antibody (anti-CEA) was immobilized on the resulted modified electrode to construct the immunosensor. The stepwise assembly process of he immunosensor was characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). CV and differential pulse voltammetry (DPV) Studies demonstrated that the formation of antibody-antigen complexes decreased peak current of [Fe(CN)(6)](3-/4-) redox pair at the AuNPs/MWCNTs-Chits/GCE. The optimization of the pH of supporting electrolyte, the incubation temperature and time were studied in detail. Under optimal conditions, the peak current of DPV of the immunosensor decreased linearly with increasing CEA concentration in two ranges of 0.3-2.5 and 2.5-20 ng mL(-1), with a detection limit of 0.01 ng mL(-1) (S/N = 3). This electrochemical immunoassay combines the specificity of the immunological reaction with the sensitivity of the AuNPs and MWCNTs amplified electrochemical detection. It would be valuable for diagnosis and monitoring of carcinoma. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available