Journal
ANALYTICA CHIMICA ACTA
Volume 665, Issue 1, Pages 98-104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.03.020
Keywords
Magnetic particles; Carcinoembryonic antigen; Gold nanoparticle; Immunoassay; Layered assembly
Categories
Funding
- National Science Foundation of China [20665003]
- Ministry of Education of China [207087]
- Natural Science Foundation of Guangxi Province [0728214]
- Guangxi Graduate Education [2007105960703M11]
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A quick and reproducible electrochemical-based immunosensor technique, using magnetic core/shell particles that are coated with self-assembled multilayer of nanogold, has been developed. Magnetic particles that are structured from Au/Fe3O4 core-shells were prepared and aminated after a reaction between gold and thiourea, and additional multilayered coatings of gold nanoparticles were assembled on the surface of the core/shell particles. The carcinoembryonic antibody (anti-CEA) was immobilized on the modified magnetic particles, which were then attached on the surface of solid paraffin carbon paste electrode (SPCE) by an external magnetic field. This is an assembly of a novel immuno biosensor for carcinoembryonic antigen (CEA). The sensitivity and response features of this immunoassay are significantly affected by the surface area and the biological compatibility of the multilayered nanogold. The linear range for the detection of CEA was from 0.005 to 50 ng mL(-1) and the limit of detection (LOD) was 0.001 ng mL(-1). The LOD is approximately 500 times more sensitive than that of the traditional enzyme-linked immunosorbent assay for CEA detection. (C) 2010 Elsevier B.V. All rights reserved.
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