Journal
JOURNAL OF VIROLOGY
Volume 81, Issue 17, Pages 9605-9608Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00635-07
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Funding
- NIAID NIH HHS [R21 AI058979, R21 AI58979] Funding Source: Medline
- NINDS NIH HHS [R01 NS046478] Funding Source: Medline
- PHS HHS [N01 A125491] Funding Source: Medline
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Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro. Normal brains from deer, transgenic mice expressing cervid PrPC [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrPC substrate produced > 6.5 X 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrPCWD is a significant step toward detection of PrPCWD in the body fluids or excreta of CWD-susceptible species.
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