Rapid simultaneous analysis of 1-hydroxypyrene, 2-hydroxyfluorene, 9-hydroxyphenanthrene, 1-and 2-naphthol in urine by first derivative synchronous fluorescence spectrometry using Tween-20 as a sensitizer

A novel method of first derivative synchronous fluorescence was developed for the rapid simultaneous analysis of trace 1-hydroxypyrene (1-OHP), 1-naphthol (1-NAP), 2-naphthol (2-NAP), 9-hydroxyphenanthrene (9-OHPe) and 2-hydroxyfluorene (2-OHFlu) in human urine. Only one single scan was needed for quantitative determination of five compounds simultaneously when Delta lambda = 10 nm was chosen. In the optimal experimental conditions, there was a linear relationship between the fluorescence intensity and the concentration of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in the range of 1.75 x 10(-9) to 4.50 x 10(-6) mol L-1, 3.64 x 10(-8) to 2.20 x 10(-4) mol L-1, 8.18 x 10(-9) to 1.20 x 10(-4) mol L-1, 3.26 x 10(-9) to 8.50 x 10(-1) mol L-1 and 4.88 x 10(-9) to 5.50 x 10(-6) mol L-1, respectively. The limits of detection (LOD) were found to be 5.25 x 10(-10) mol L-1 for 1-OHP, 1.10 x 10(-8) mol L-1 for 1-NAP, 2.46 x 10(-9) mol L-1 for 2-NAP, 9.77 x 10(-10) mol L-1 for 9-OHPe and 1.46 x 10(-9) mol L-1 for 2-OHFlu. The proposed method is reliable, selective and sensitive, and has been used successfully in the determination of traces of 1-OHP, 1-NAP, 2-NAP, 9-OHPe and 2-OHFlu in human urine samples, whose results were in good agreement with those gained by the HPLC method. (C) 2009 Elsevier B.V. All rights reserved.