Journal
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Volume 133, Issue 9, Pages 599-611Publisher
SPRINGER
DOI: 10.1007/s00432-007-0206-0
Keywords
carbohydrate; enzyme activities; gastric tumor; glycosyltransferases; sulfotransferases
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Funding
- NCI NIH HHS [CA160561, CA35329] Funding Source: Medline
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Purpose Several reports indicate a complexity in glycosyltransferase activities which lead to several tumor associated carbohydrate structures in gastric carcinoma. The present study was aimed to identify the carbohydrate associated transferases which exhibit the most marked and consistent change of activity in gastric tumorigenesis. Methods We examined the levels of fucosyl, beta-galactosyl-, beta-N-acetylgalactosaminyl, sialyl- and glycan:sulfotransferase activities, which generate the outer ends of oligosaccharide chains in tumorous and adjacent normal gastric tissues of the same patient in ten gastric carcinoma cases by using well defined specific synthetic acceptors utilized in our several earlier published studies as referenced in the text (e.g. Chandrasekaran et al. in J Biol Chem 279:10032-10041, 2004; Biochemistry 44:15619-15635, 2005; Carbohydr Res 341:983-994, 2006). Results Among glycosyltransferases only alpha 1,2-fucosyltransferase (FT) was unique in showing a remarkable 40-90% decrease of activity in seven cases. Uniquely several fold elevation of Gal3Sulfo-T-2 (1.9 -> 156.7 fold) and Gal3Sulfo-T-4 (2.4 -> 149.0 fold) activities in all ten cases and moderate elevation of GlcNAc6Sulfo-T (1.3 -> 37.5 fold) activities in nine cases were identified. Poorly differentiated Signet ring cell carcinoma expresses mainly Gal3Sulfo-T-2 activity whereas poorly differentiated adenocarcinoma express predominantly Gal3Sulfo-T-4 activity and also GlcNAc6Sulfo-T activity. But, very low level of these sulfotransferase activities were identified in moderately differentiated gastric carcinomas as well as non-epithelial gastric stromal sarcoma. Conclusion Up regulation of glycan:sulfotransferase activities and down regulation of alpha 1,2-fucosyltransferase activity are apparently associated with human gastric tumorigenesis.
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