Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 196, Issue 5, Pages 796-800Publisher
OXFORD UNIV PRESS INC
DOI: 10.1086/520536
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Funding
- NIAID NIH HHS [R01 AI066087-03, R01 AI066087, R01 AI025780, R01 AI025780-21, R01 AI25780, R01 AI025780-19, R01 AI025780-20, R01 AI066087-01, R01 AI066087-02, R01 AI025780-22, R01 AI37532] Funding Source: Medline
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Mannoproteins are major antigens driving T cell responses to the opportunistic fungus Cryptococcus neoformans. To investigate the role played by mannosylation, an immunoreactive cryptococcal mannoprotein was expressed recombinantly in E. coli and Pichia pastoris, resulting in unglycosylated and mannosylated proteins, respectively. The Pichia-derived antigen stimulated stronger major histocompatibility class II-restricted T cell responses. Moreover, responses were potently inhibited if the antigen was chemically deglycosylated or if mannose receptors were blocked with mannans. Thus, mannosylation is critical for optimal T cell responses to a fungal antigen and should be taken into account when vaccines to protect against mycoses are designed.
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