Journal
ANALYTICA CHIMICA ACTA
Volume 655, Issue 1-2, Pages 38-42Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2009.10.001
Keywords
Multivariate curve resolution; Alternating least squares; Second order advantage; Dyes; Beverages samples
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Funding
- Universidad Nacional del Sur
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A direct spectrophotometric method for the determination of three artificial colors - amaranth, sunset yellow FCF and tartrazine - in beverages samples is proposed. The spectra were recorded between 359 and 600 nm. The spectra of the samples (just filtrated), pure dyes (concentrations ranged between 0.01 and 1.8 mg L-1 for amaranth, 0.08 and 4.4 mg L-1 for sunset yellow and 0.04 and 1.8 mg L-1 for tartrazine) and synthetic mixtures were disposed in a column-wise augmented data matrix. This kind of data structure, analyzed by multivariate curve resolution-alternating least squares (MCR-ALS) makes it possible to exploit the so called 'second order advantage'. MCR-ALS algorithm was applied to the experimental data under the non-negativity and equality constraints. As a result, the concentration of each dye in the samples and their corresponding pure spectra were obtained. The results were validated using internal reference materials and no significant differences were found (alpha = 5%) between the reference values and the ones obtained with the proposed method. The second order advantage made it possible to obtain unbiased results even in the presence of interferences. m 2009 Elsevier B.V. All rights reserved.
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