Journal
ANALYTICA CHIMICA ACTA
Volume 650, Issue 2, Pages 151-159Publisher
ELSEVIER
DOI: 10.1016/j.aca.2009.07.034
Keywords
Proteomics; Protein identification; Mass spectrometry; Fast methods
Categories
Funding
- FCT (Science and Technological Foundation) from Portugal [SRFH/BD/38509/2007, SFRH/BD/28563/2006, SFRH/BD/31652/2007, SFRH/BD/38504/2007]
- Xunta de Galicia (Spain)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/38504/2007, SFRH/BD/28563/2006] Funding Source: FCT
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Recent tools addressed to accelerate the different steps of the sample treatment for protein identification in modern workflows are reviewed and critically commented in this manuscript. Heating, microspin columns, ultrasonic energy, high pressure, infrared energy, microwave energy, alternating electric fields and microreactors are outlined as useful tools that can be used to accelerate all or some of the following steps for in-gel or in-liquid based approaches for protein identification: (i) protein dissolution/denaturation, (ii) protein reduction, (iii) protein alkylation and (iv) protein digestion. The advantages and drawbacks, along with the main differences among the different tools are also commented. Future prospects for hyphenation of methods are also discussed. Researchers are informed also in this work regarding the main problems to be found when implementing any of the above mentioned methods. (C) 2009 Elsevier B.V. All rights reserved.
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