Journal
ANALYTICA CHIMICA ACTA
Volume 639, Issue 1-2, Pages 96-100Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2009.02.047
Keywords
Chemiluminescence; Tetracyclines determination; Fenton system; Eu(III) ion
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A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(Ill) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4 x 10(-7) to 2 x 10(-5) mol L-1 of Chlor-TC; 2 x 10(-7) to 2 x 10(-5) mol L-1 of Oxy-TC and 1 x 10(-7) to 3 x 10(-1) mol L-1 of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey. (C) 2009 Elsevier B.V. All rights reserved.
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