Journal
ANALYTICA CHIMICA ACTA
Volume 633, Issue 2, Pages 244-249Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.11.068
Keywords
Amperometric immunosensor; Fc-COOH-doped silica nanoparticles; Cancer antigen 15-3
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Funding
- NNSF of China [20675064]
- NSF of Chongqing City [CSTC-2004BB4149, 2005BB4100]
- Key Laboratory of MOE on Luminescence and Real-Time Analysis [2006CA8006]
- High Technology Project Foundation of SWU, China [XSGX02]
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The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of Fc-COOH and were easily modified with trialkoxysilane reagents for covalent conjugation of CA 15-3 antibodies (anti-CA 15-3). The Fc-COOH-doped SNPs were characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The fabrication process of the electrochemical immunosensor was demonstrated by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Under optimal conditions, the developed immunosensor showed good linearity at the studied concentration range of 2.0-240 U mL(-1) with a coefficient 0.9986 and a detection limit of 0.64 U mL(-1) at S/N=3. (C) 2008 Elsevier B.V. All rights reserved.
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