Journal
DIGESTIVE DISEASES AND SCIENCES
Volume 52, Issue 9, Pages 2029-2036Publisher
SPRINGER
DOI: 10.1007/s10620-006-9449-x
Keywords
polymeric formulae; inflammatory bowel disease; anti-inflammatory effects; in vitro model
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Exclusive enteral nutrition using polymeric formula (PF) is a well-established therapeutic option for active Crohn's disease; however, its mechanisms of action are unknown. We investigated the anti-inflammatory effects of PF in an in vitro model of epithelial cell inflammation. PF did not affect cell viability over a range of dilutions, but when PF was added to the culture medium the interleukin (IL)-8 response to proinflammatory stimuli was significantly reduced. This effect was due to PF acting directly on the cells as the IL-8 response was still reduced when PF was separated from the proinflammatory stimuli in a 2-compartment system. In the presence of PF, nuclear factor (NF)-kappa B nuclear migration was not inhibited; however, I kappa B alpha degradation was delayed. PF has direct anti-inflammatory effects upon immortalized colonic enterocytes. Therefore PF may, in part, modulate gut inflammation by directly reducing the inflammatory response of the intestinal epithelium.
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