Journal
CLINICS IN LABORATORY MEDICINE
Volume 27, Issue 3, Pages 653-+Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.cll.2007.05.008
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Funding
- NCI NIH HHS [9 R44 CA01798-02, R44 CA001798-02] Funding Source: Medline
- NIGMS NIH HHS [R43 GM058956-01, 1 R43 GM58956-01] Funding Source: Medline
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Imaging flow cytometry combines the statistical power and fluorescence sensitivity of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. Examples are provided of the discrimination of cancerous from normal mammary epithelial cells and the high-throughput quantitation of fluorescence in situ hybridization (FISH) probes in human peripheral blood mononuclear cells. The FISH application will be enhanced further by the integration of extended depth-of-field imaging technology with the current optical system.
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