Journal
ANALYTICA CHIMICA ACTA
Volume 638, Issue 1, Pages 51-57Publisher
ELSEVIER
DOI: 10.1016/j.aca.2009.01.063
Keywords
Phosphorylation; Phosphopeptide enrichment; Metal oxide affinity chromatography; Tin dioxide; Mass spectrometry; Nanocasting
Categories
Funding
- GEN-AU (Genome Research in Austria)
- Austrian Federal Ministry of Science and Research
- European Union Sixth Framework Program
Ask authors/readers for more resources
Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO2 microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be Successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters. (c) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available