Journal
ANALYTICA CHIMICA ACTA
Volume 650, Issue 1, Pages 10-15Publisher
ELSEVIER
DOI: 10.1016/j.aca.2009.01.054
Keywords
Hydrophilic interaction liquid chromatography; Plasma; Metabonomics; Highly polar metabolites
Categories
Funding
- Natural Science Foundation of China [20775079]
- DICR CAS [K 2006A3]
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Highly polar metabolites are abundant in human plasma. Analysis of these compounds by standard reverse-phase chromatography is difficult. Highly polar metabolites were extracted by a two-step method. They were analyzed by ultra-performance hydrophilic interaction chromatography (HILIC) coupled to quadrupole-time of flight mass spectrometer detection technology. The middle and low polar fraction were also analyzed using a standard C18 column. Data were extracted by Markerlynx, and analyzed by multivariate statistical methods to discriminate human plasma samples by sex and age. The results not only showed good classification of samples by sex and age, but also revealed new potential biomarkers not revealed by standard RPLC/MS analysis. This study showed that HILIC is an effective technique to analyze highly polar metabolites for metabonomic studies. (C) 2009 Elsevier B.V. All rights reserved.
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