Journal
ANALYTICA CHIMICA ACTA
Volume 633, Issue 1, Pages 97-102Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.11.031
Keywords
Supramolecular assembly; Fluorescence sensor; Oligonucleotide ligand; Molecular interaction
Categories
Funding
- NSF of China [20775082, 90717119]
- 973 Program [2007CB935601]
- 863 Program [2007AA02Z221]
- Center for Molecular Science, Institute of Chemistry, CAS
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This paper describes a universal, label-free fluorescence sensor that gauges the interaction of oligonucleotides with their targets. The sensor is based on supramolecular assemblies formed by oligonucleotides (polyanions) and small molecule cation surfactant in aqueous solution. The environmentally dependent dye pyrene, encapsulated in the apolar interiors of the assemblies via hydrophobic interactions works as the fluorescence probe. Target binding causes the conformational change of the oligonucleotides, which results in disorganization of supramolecular assemblies, release of pyrene into the aqueous solution and subsequent quenching of its fluorescence. The kinetic processes (including the formation of supramolecular assemblies and the release of pyrenes after adding the targets) were investigated. The fluorescence decreases of pyrenes are proportional to the concentrations of targets within the linear ranges. This label-free fluorescence system is simple,convenient, low cost, and can serve as an alternative tool for interaction studies of oligonucleotides with their targets, especially with small molecular targets. (C) 2008 Elsevier B.V. All rights reserved.
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