Journal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Volume 48, Issue 9, Pages 4153-4161Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.07-0251
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- NEI NIH HHS [R01 EY017373-02, R24 EY015636, R01 EY014366-06, EY017373, R01 EY014366, R01 EY017373, EY014366] Funding Source: Medline
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PURPOSE. The aims of this study were to determine whether IL-17(+) T cells were present in CD4 and CD8 interphotoreceptor retinoid-binding protein ( IRBP)-specific T cells and to determine the role of antigen-specific and nonspecific IL-17 (+) T cells in the pathogenesis of experimental autoimmune uveitis (EAU). METHODS. B6 mice were immunized with uveitogenic peptide IRBP1-20. In vivo-primed T cells were separated and stimulated with the immunizing peptide. Intracellular expression of IFN-gamma and IL- 17 by the T cells was assessed, and the pathogenic activity of the activated T cells was determined. RESULTS. A subset of autoreactive IRBP-specific CD8 T cells expressed IL-17. IRBP-specific T cells preferentially expressed IL-17 when expanded by IL- 23, whereas IFN-gamma -expressing cells were dominant when the T cells were cultured with IL-2. Importantly, both expanded T-cell populations were uveitogenic. In addition, IL-23 promoted the expansion of antigenspecific and non-antigen-specific IL-17(+) T cells, whereas TGF-beta and IL-6 acted only on non-antigen- specific IL-17(+) T cells. Only the antigen-specific IL-17(+) T cells were uveitogenic. The activation of autoreactive IL-17(+) T cells was markedly increased in vivo by the mycobacterial component of CFA and pertussis toxin ( PTX) and in vitro by the ligation of Toll-like receptors. CONCLUSIONS. IL-17(+) T cells can be readily detected among activated autoreactive and bystander T cells and may play a major role in the pathogenesis of EAU.
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