Headspace SPME-GC method for acetone analysis and its biomedical application

A new method for extraction and analysis of acetone in human urine based on headspace solid phase microextraction using a mixture of activated carbon and zeolite as sorbents in a PVC matrix coated on a silver wire and its application to the determination of ketone bodies is described. Unlike commercial fibers, which are coated on fused silica, the coating adheres strongly to the silver wire and is thermally stable up to 250 degrees C. After optimization of coating composition and microextraction conditions the Fiber was used for the analysis of acetone in human urine.