Journal
ANALYTICA CHIMICA ACTA
Volume 630, Issue 2, Pages 168-173Publisher
ELSEVIER
DOI: 10.1016/j.aca.2008.10.001
Keywords
Peptide nucleic acid; Surface plasmon resonance; 16s rRNA; Cationic nanoparticle
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Funding
- Ministry of Health & Welfare, Republic of Korea [03-PJ1-PG1-CH11-0003]
- Ministry of Science & Technology (MOST)
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A signal enhancing method allowing highly sensitive detection of E. coli 16s rRNA was developed using peptide nucleic acid (PNA) as a capture probe and a surface plasmon resonance (SPR) sensor as a detector. 16s rRNA has been used as a genetic marker for identification of organisms, and can be analyzed directly without PCR amplification due to the relatively high number of copies. PNA has a neutral backbone structure, therefore hybridization with 16s rRNA results in the ionic condition being changed from neutral to negative. A cationic Au nanoparticle was synthesized and used for signal amplification by ionic interaction with 16s rRNA hybridized on the PNA probe-immobilized SPR sensor chip. This method resulted in a detection limit of E. coli rRNA of 58.2 +/- 1.37 pg mL(-1). Using this analytical method, Staphylococcus aureus was detected without purification of rRNA. (C) 2008 Elsevier B.V. All rights reserved.
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