Home-made capillary array electrophoresis for high-throughput amino acid analysis

A capillary array electrophoresis (CAE) with confocal rotary scanner for high-throughput carboxytetramethy1rhodamine succinimidyl ester (TAMRA)-labeled amino acid (AA) analysis is presented. Performance of the CAE setup was evaluated with AA samples. Up to 128 capillaries could be detected in parallel. For the first time, the device was applied to separate the enantiomers of isoleucine, cysteine, and glutamic acid with cyclodextrinmodified electrolytes by capillary zone electrophoresis. Baseline separation of seven AAs is also demonstrated using micellar electrokinetic chromatography method. (c) 2008 Elsevier B.V. All rights reserved.