Journal
ANALYST
Volume 143, Issue 18, Pages 4484-4494Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7an01990d
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Funding
- European Union's Seventh Framework Programme for research, technological development and demonstration [608847]
- R'EQUIP grant from the Swiss National Foundation [326030-157759]
- Faculty of Medicine (University of Bern)
- Swiss National Science Foundation (SNF) [326030_157759] Funding Source: Swiss National Science Foundation (SNF)
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Steroids are key players in a high variety of physiological processes and are typically analyzed for the diagnosis of hormonal disorders. Due to their chemical and structural similarity many of these metabolites cannot be separated by conventional techniques such as liquid chromatography. Herein, we present an analysis strategy based on two dimensional gas chromatography (GCxGC) coupled to time-of-flight mass spectrometry (TOF MS) which demonstrates superior separation power and enables comprehensive screening of steroids. We show absolute quantitation of 40 steroids in human urine over three orders of magnitude with limits of detection 50 nM and the tentative identification of additional 30 steroids based on accurate mass, isotopic pattern analysis and spectral similarity matching to known steroids. The method displays excellent inter- and intra-day stability, repeatability and recovery and was validated for clinical routine analysis. Additionally, we demonstrate the potential of the approach for untargeted analysis of urinary steroids in mouse and rat.
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