Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 9, Pages 3393-3399Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr0702518
Keywords
in-solution protein digestion; protein identification; ultrasonic probe; sonoreactor; ultrasonic bath; MALDI-TOF-MS
Categories
Ask authors/readers for more resources
Three ultrasonic energy sources were studied to speed up the sample treatment for in-solution protein identification by peptide mass fingerprint using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein reduction, alkylation, and enzymatic digestion steps were done in 15 min. Nine proteins, including zinc resistance-associated protein precursor from Desulfovibrio desulfuricans strain G20 and split-soret cytochrome c from D. desulfuricans ATCC27774 were successfully identified with the new protocol.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available