Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids

Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs-gold nanoparticle (SWCNT-GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A targeted photothermolysis experiment using 670 nm light at 2 W cm(-2) for 15 min, resulted in selective and irreparable damage to more than 99% Salmonella DT104 at the concentration of 105 CFU mL(-1). In comparison to solely SWCNTs or GNPs, our SWCNT-GNPs nanohybrids have also shown a better photothermal efficiency.